Biosafety'

Would any of your project ideas raise safety issues?

No issues of researcher safety, public safety or environmental safety were raised during Heidelberg's iGEM 2009 project. We only worked with non-hazardous, non-infectious, commonly used and accepted bacteria strains (SCS110 and DH5α) and mammalian cancer cell lines (U2-OS, HeLa and MCF-7). When working with toxic chemicals (e.g. ethidiumbromide or estrogen), nitrile gloves, and white coat were worn. We specifically reserved an area of our lab for work with ethidiumbromide. Work with other toxic chemicals (for cell culture work) was done under a HERA Safe hood. All work was conducted in a biosafety level S1 laboratory. Rules of best microbiological practices were applied.

Is there a local biosafety group, committee or review board at your institution? According to German Federal law, there is a project leader for biological safety issues for the group that advised us, who was glad to take the responsibility for our project's saftey issues also (M. Reichenzeller, PhD m.reichenzeller at dkfz-heidelberg.de). There also is a biosaftey supervisor who supervises biological saftey university-wide (W. Siller, PhD willi.siller at zuv.uni-heidelberg.de)

What does your local biosafety group think about your project? All material handled or distributed are non-hazardous and non-infectious. It agrees with all safety standards requested biosafety level 1. Therefore, Dr. Reichenzeller fully supports the work done by this iGEM team.

Do any of the new BioBrick parts that you made this year raise any safety issues?

No - we developed synthetic promoters for use in mammalian cells. These parts are completely harmless, no matter what organism they are transformed / transfected into.