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From 2009.igem.org

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	LB medium DNA resuspension from iGEM 2009 plates Transformation X-Gal staining protocol for beta galactosidase (blue/white screening) Glycerol stocks Plasmid extraction BioBrick digestion with restriction enzymes Electrophoresis DNA gel extraction DNA precipitation with sodium acetate Ligation PCR Sensing ethanol concentration: our do-it-yourself kit

LB medium

(Estimated time: 10 min + 4 hours of autoclavation and cooling)

For a final volume of 1 l, put: 10 g NaCl 10 g BactoTryptone 5 g Yeast extract 15 g Agar (only for LB plates) into 1 l of ddH2O. Autoclave the solution. Let it cool to ~40-45°C Add the proper antibiotic if needed. ONLY FOR PLATES: Pour an homogenous layer of agar LB into Petri plates under the hood. Let agar LB polymerase. Cover and store at +4°C. Always check for contaminations before using!

Clean bottle or flask NaCl BactoTryptone Yeast extract ddH2O Agar

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