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Team:Imperial College London/M2/EncapsulationRationale
From 2009.igem.org
Contents |
Module 2 - Acid Resistance Overview
The need for encapsulation:
While E.coli has endogenous acid resistance pathways, colonisation of the gut is based on a "numbers approach". In essence, the majority of E.coli cells in a population do not survive passage through the stomach but the few that do are able to regenerate the population once in the intestine.
This point is illustrated by the experiment shown below:
Constitutive GFP producing E.coli cells were exposed to differing concentrations of acid for 30 minutes. The reduction in fluoresence is indicative of cell lysis and the subsequent acid-induced denaturing of GFP.
To tackle this seemingly insurmountable problem we adopted a two phase approach.
Phase 1: Identify a suitable chassis with the genotypic potential for acid–resistance.
Phase 2:Manipulate endogenous acid resistance pathways to control the acid resistant phenotype.
Phase 1:
Our rationale for looking for natural sources of acid resistance is that it is easier to hack existing pathways than to transfer large numbers of genes into a different chassis with a dissimilar genetic background.
Based on natural sources of acid resistance, Lactobacillus, E.coli and B.subtilis were shortlisted as potential chassis.
Of these three organisms, E.coli was chosen as it is safe, easy to work with and possesses a broad range of acid resistance strategies.
Phase 2:
We hacked E.coli’s acid resistance module in three places to achieve the production of a safe acid resistant capsule.
Acid Resistant Polymer – Colanic acid: E.coli naturally produces a harmless acid–resistant polymer known as colanic acid. Colanic acid is a polymer of glucose, galactose and glucuronic acid. By tapping into the pathway that initiates colanic acid biosynthesis, we can turn on its production via the modulation of a transcription factor encoded by a gene called RcsB.
About RcsB
Safety – Biofilm prevention:
In nature, colanic acid acts as a binding agent between individual cells over which a biofilm can be formed. While colanic acid itself is harmless, biofilm formation is associated with a number of virulence factors. To prevent biofilm formation from occurring, we have tapped into a second pathway such that our cells become locked into colanic acid production. The gene responsible for preventing biofilm formation is a transcription factor encoded by a gene called YgiV.
About YgiV
Microencapsulation – Colanic acid tethering:
In nature, colanic acid is associated with but not attached to the cell surface. To facilitate whole cell encapsulation, we have modified a third pathway to fix the colanic acid to the surface of the cell. This involves the over–production of an enzyme called Rfal.
About Rfal
Module 2 - Encapsulation
