Team:Todai-Tokyo/Protocols/Notebook Sample

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Contents

1 Miniprep
2 PCR
3 Sequencing
4 Transformation
5 Ligation
6 Infusion
7 Gel Purification
8 RE Digest

Miniprep

The following were miniprepped:

pLacI-RFP-dterm (From Ligation on 9/4; 4 colonies)
RFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450 BBa_J04450] from [http://partsregistry.org/assembly/libraries.cgi?id=192009 Spring Distribution]; 6 colonies)

PCR

Sequencing

Transformation

Ligation

Infusion

Gel Purification

RE Digest

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