Transformation
5 mL Overnights
Glycerol Stock
Plasmid Miniprep
Restriction Digest
Agarose Gel Electrophoresis
Gel Extraction
Ligation
Colony PCR
Vector Dephosphorylation
Sanger Sequencing Reaction
Byte Amplification and Prep
Byte Assembly (in a tube)
Byte Construct Linearization)