Team:Alberta/Project/AnchTermAnneal

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University of Alberta - BioBytes










































































































Annealing Anchor and Terminator Bytes

Instructions on how to anneal the complementary strands to obtain the same concentrations we used in our experiments (10uM).

What you will need:

  • Anchor oligos (complementary)
  • Terminator oligos (complementary)

Procedure:

  • Pipet 150 uL 50% glycerol into 3* properly labeled 1.5 ml screw cap tubes.
  • Add 350 uL of overnight culture to each tube.
  • Pipet up and down to gently mix.
  • Flash freeze in liquid N2 or dry ice/methanol bath
  • Place in -80 C freezer when frozen.

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