Team:Todai-Tokyo/Protocols/Miniprep

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Miniprep Protocol

Preparation (previous night)

  1. Pick Single colonies from a transformation plate and culture in 4ml Luria Broth
  2. Culture overnight at 37ºC with vigorous shaking

Miniprep

  1. Aliquot 2ml of the bacterial suspension from above into a 2ml eppendorf
  2. Spin down for 5 min. at max speed to pellet cells
  3. Use the Promega miniprep kit according to instructions

We re-suspend the DNA pellet in 50µl MilliQ and measure nucleotide concentration on a spectrophotometer to label on the tube.

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