Team:VictoriaBC/Design

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DESIGN
The final project consisted of a simplified biothermometer design. Using the 32°C ribothermometer linked to either RFP or GFP would entail a system that responded visually to differences in temperature. There were two levels for this: K235034 and K235035 used a lactose promoter and repressor to regulate RFP and GFP respectively; and K235036 and K235037 were directly coupled to RFP or GFP respectively.
K235034
K235034 (Chl) const. promoter+32oC temp. sensing RBS K235033(Kan) const. promoter+32oC temp. sensing RBS J04450(Kan) Lac controlled RFP P1010-Chl

No

          
K235035 (Chl) const. promoter+32oC temp. sensing RBS+ lactose promoter+RBS+GFP+stop K235033(Kan) const. promoter+32oC temp. sensing RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl No          
K235036 (Kan) const. promoter+32oC temp. sensing RBS+RFP+stop K235030(Chl) const. promoter+32oC temp. sensing RBS K235005(Chl) RFP+stop P1010-Kan Assembled Colonies Band -20oC    
K235037 (Chl) const. promoter+32oC temp. sensing RBS+GFP+stop K235030(Kan) const. promoter+32oC temp. sensing RBS J04630(Amp/Kan) GFP+stop P1010-Chl Assembled Colonies Band -20oC    
K235038 (Chl) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Chl            
K235038 (Kan) const. promoter+RBS J23102(Amp) const. promoter B0034 (Amp) RBS P1010-Kan            
K235039 (Kan) const. promoter+RBS+LacI repressor+LVA+stop K235038 (Chl) const. promoter+RBS K235031 (Chl) LacI repressor+LVA+stop P1010-Kan            
K235040 (Chl) const. promoter+RBS+Lac controlled RFP K235039 (Kan) const. promoter+RBS J04450(Kan) Lac controlled RFP P1010-Chl            
K235041 (Chl) const. promoter+RBS+lactose promoter+RBS+GFP+stop K235039 (Kan) const. promoter+RBS K235032(Kan) lactose promoter+RBS+GFP+stop P1010-Chl            
K235042 (Kan) const. promoter+RBS+RFP+stop K235038 (Chl) const. promoter+RBS K235005(Chl) RFP+stop P1010-Kan            
K235043 (Chl) const. promoter+RBS+GFP+stop K235038 (Kan) const. promoter+RBS J04630(Amp/Kan) GFP+stop P1010-Chl            
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