Team:TorontoMaRSDiscovery/8 August 2009
From 2009.igem.org
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August 8, 2009
- BB1 transformants showed many colonies
- plates (a plate full of colonies)
- -> increasing heat shock to 90s increased efficiency (Note: 3x DNA used in this transformation)
- Calvin mentioned he does heat shock for 120s
- Calvin also suggested to increase tranformation time, ie. after adding DNA, hold on ice for an hour
- DH-5alpha replates all grew on the plates, demonstrating LB can be re-autoclaved and poured as plates
- there was no difference between transformants directly plated after adding LB+glucose or incubating for an hour
- Miniprepped overnights (1+2) samle 1,2,4, and K