Team:TorontoMaRSDiscovery/31 August 2009
From 2009.igem.org
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August 31, 2009
- Stocked 4+5R culture (retransfection)
- Miniprepped overnight cultures of 4+5 (3,4,5,R), BB7, and EncX,Y,Z
- Digested EncC with E,S; 5 with X,P; Tet, 7, EncX,Y,Z with E,P
- Ran digests on a gel
- EncY shows Enc insert
- 5 digest lane shows a thick band the same size of the linear isoform in the control
- Poured C plates
- 3+2 tranformation from Aug 27 showed a few colonies
- 3 largest colonies were picked to start overnight culture
- Started overnight ligation of Enc+5/Tet with negative control