Team:TorontoMaRSDiscovery/31 August 2009

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August 31, 2009

  1. Stocked 4+5R culture (retransfection)
  2. Miniprepped overnight cultures of 4+5 (3,4,5,R), BB7, and EncX,Y,Z
  3. Digested EncC with E,S; 5 with X,P; Tet, 7, EncX,Y,Z with E,P
  4. Ran digests on a gel
    • EncY shows Enc insert
    • 5 digest lane shows a thick band the same size of the linear isoform in the control
  5. Poured C plates
  6. 3+2 tranformation from Aug 27 showed a few colonies
  7. 3 largest colonies were picked to start overnight culture
  8. Started overnight ligation of Enc+5/Tet with negative control
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