Team:Wisconsin-Madison/Ligation

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Ligation Concentration 1 backbone : 5 insert insert: [100 x (insert size bp/backbone size bp) x 5 / concentration(ng)] μL Thermocycler users: Dan A: Lig 2 hours 16 C or overnight Materials 1μL T4 ligase 1 μL T4 ligase buffer backbone + insert =< 8μL (remainder H20) Back to Protocols