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Team:Wisconsin-Madison/Ligation
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Revision as of 18:04, 20 October 2009 by
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Ligation
Concentration
1 backbone : 5 insert
insert: [100 x (insert size bp/backbone size bp) x 5 / concentration(ng)] μL
Thermocycler
users: Dan A: Lig
2 hours 16 C or overnight
Materials
1μL T4 ligase
1 μL T4 ligase buffer
backbone + insert =< 8μL (remainder H20)
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