Protocols

Cell culturing/cloning protocols

• CC1: Miniprep
• CC2: Midiprep
• CC3: Making cells competent
• CC4: Ligation
• CC5: Transformation into BL21 using electrical shock
• CC6: Transformation into Top10 using chemical competence
• CC7: Making LB plates
• CC8: Extracting DNA from the registry
• CC9: Preparation of XL1-Blue electrocompetent cells & XL1-Blue quality control
• CC10: PCR
• CC11: SLIC
• CC12: Biobricking parts from oligos
• CC13: Genome Prep for Restriction Assay

Calibrations

• CA1: Optical Density Calibration
• GFP fluorescence calibration
  • CA2: External GFP fluorescence
  • CA3: Intracellular GFP fluorescence

Promoter Characterisation

• PP1: Lac Promoter Characterisation

Autoinduction

• AI1: Secondary Carbon Source Experiment
• AI2: Glucose Time Delay

Protein Production

• PP1: IPTG Toxicity
• PP2: IPTG characterisation
• PP3: Cellulase
• PP4: PAH

Encapsulation

• EN1: Colanic acid production amount
• EN2: Colanic acid protection from pH
• EN3: Trehalose production

Killing

• KI1: Thermoinduction
• KI2: Restriction Enzyme activity