August/1 October 2009

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1.colony check

sample no.      no. of colony
    41                       +++
    42                       +++
    48                       +++
   1-1K                    +++
   1-6I                     +++
     57                       +

2.min prep

sample no.         conc.
       52                61.4 ng/uL

3.fluorence check (color parts)

 not confirm fluorence...

4.prepared LA plate

5.transformation

sample
1-14N , 1-16I

6.digation

K204058

Vector		Insert
1-8E	4	1-8I	10
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	12	dH₂O	6
total	20uL	total	20uL

K204059

Vector		Insert
1-8E	9	1-8K	2
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	7	dH₂O	14
total	20uL	total	20uL

K204060

Vector		Insert
1-8E	8	1-12M	4
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	8	dH₂O	12
total	20uL	total	20uL

K204061

Vector		Insert
1-8E	8	2-8E	8
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	8	dH₂O	12
total	20uL	total	20uL

K204062

Vector		Insert
1-8E	9	33	1
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	7	dH₂O	15
total	20uL	total	20uL

K204063

Vector		Insert
1-23L	6	1-4E	9
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	2	No.2	2
dH₂O	10	dH₂O	7
total	20uL	total	20uL

↓
37°C, O/N

7.min prep

sample       conc.
   X1            261.1 ng/uL
   X2           301.5
   X3           345.9
   X4           360
   X5           192
   X6           219
   X7           319
   X8          353
   X4          334

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