August/13 October 2009

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1.colony check

sample     no. of colony
  67             ++
  68             ++
  71             +
  73             +
  79             + 
  80             +
  81             +
  82             +
  83             +
  84             +

2.digation
K204072

Vector		Insert
2-8E	5	31	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	33	dH₂O	33
total	50uL	total	50uL

K204090

Vector		Insert
1-23L	5	chiba 3	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	33	dH₂O	33
total	50uL	total	50uL

37degree , 2hr
gel cut& purification
ligation

3.min prep

sample    conc.
 68           53 ng/uL
                142
                 76
                 59

4.digation

K204091

Vector		Insert
65	5	76	5
EcoRI	1	EcoRI	1
XbaI	1	SpeI	1
No.2	5	No.2	5
dH₂O	33	dH₂O	33
total	50uL	total	50uL

K204092

Vector		Insert
77	3	65	10
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	35	dH₂O	38
total	50uL	total	50uL

K204093

Vector		Insert
76	2	67	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	32
total	50uL	total	50uL

K204094

Vector		Insert
77	3	67	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	35	dH₂O	32
total	50uL	total	50uL

K204095

Vector		Insert
76	2	1-19B	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	32
total	50uL	total	50uL

K204096

Vector		Insert
77	2	1-19B	6
SpeI	1	XbaI	1
PstI	1	PstI	1
No.2	2	No.2	2
dH₂O	36	dH₂O	32
total	50uL	total	50uL

↓
37 degree , 2hr
gel cut & purification
ligation

5.min prep

sample         conc.
 67                 87  ng/uL
 68                 28
 71                 64
 73                 12
 79                   5
 80                   9
 81                 75
 82                 14
 83                 19
 84                 15

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