Team:British Columbia/pBAD

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Arabinose sensor: the pBAD promoter

Overview

In order to make an analog biosensor, we need our traffic light to be able to respond differently to different concentrations of an input. However, the Registry is lacking in variable strength inducible promoters. We designed two variants of the pBAD promoter, one weaker and one stronger, based on AraC binding experiments performed by Niland et al.

A diagram of the changed nucleotide sequences of arabinose-inducible promoters. Green nucleotides of the wildtype show all sequences that were mutated to form strong and weak.


Above is a sequence alignment of our promoter variants with the wild type pBAD, truncated for space. For the full sequences, see our parts in the registry at (strong) and (weak).

Using these promoter sequences, we assembled XXX... Timecourse.jpg Toxicity.jpg Trans fcn.jpg

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