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Team:IIT Bombay India/Notebook
From 2009.igem.org
Week1 FACS Trial
Week2 (May, 18-23) Preparation
Week3 (May, 25-30) FACS
Week4 (Jun, 1-6) Growth Curve
Week5 (Jun, 8-13) FACS:YFP signal
Week6 (Jun, 15-20) FACS:CFP signal
Week7 (Jun, 22-27) FACS repeat
Week8 (Jun-Jul, 29-4)Req.:Transformation
Week9 (Jul, 6-11) Cultured Host
Week10(Jul, 13-18) Transformation of 4 strains
Week11(Jul, 20-24) FACS:Constructs
Week12(Jul, 27-31) FACS
Week13(Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)
Week14(Aug, 10-15) Preparation for time course expression analysis
Week15(Aug, 17-22) FACS time course
Week16(Aug, 24-29) FACS: different IPTG conc.
Week17(Aug-Sep, 31-5)Growth curves for all the four strains
Week18(Sep, 7-13) Plasmid extraction and Gel electrophoresis
Week19(Sep, 14-20) Growth curve on Lactose (ptet A and plac K)
Week20(Sep, 21-27) Growth curve on Lactose (plac A and ptet K)
Week21(Sep-Oct, 28-3)Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)
Week22(Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)
Week23(Oct, 12-17)Growth curve on Lactose (ptet A and plac K)
Week24(Oct, 18-21)Data compilation and analysis, wiki uploads
