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Duke University/16 June 2009
From 2009.igem.org
June 16, 2009
- E: PCR with Bioplastics #2 as template and Phusion mix (done by Maggie), Gels to check PCRs
2x Phusion mix 25 ul forward primer 2.5 ul reverse primer 2.5 ul DNA template (plasmid) 1 ul H2O 19 ul 50 ul
- R: No bands for fragments 3, Bands for fragment 4
- C: Cut out bands of fragment 4, PCR again to obtain fragment 3
- E: Measured concentration of DNA fragments 1 and 2
- R: Concentrations okay
- C: Do assembly PCR once enough of fragments 3 and 4 obtained
