Team:NTU-Singapore/Notebook

From 2009.igem.org

Revision as of 19:07, 21 October 2009 by Sjee (Talk | contribs)

Experiment Timeline


June

18th June

  • Preparation of LB medium
  • Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)


19th June

  • Antibiotic stock preparation
  • Inoculation


20th June

  • Our first Plasmid Extraction (PE)!


22nd June

  • First Double Digestion (DD) and Gel Check!


23rd June

  • PE & DD of the registry constructs….


24th June

  • Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).


26th June

  • PE , DD and Gel check for the direct synthesized gene sequence.


27th June

  • Gel Check for CHE, IFP and pNO


July








NTU@iGEMcc 2009. Some rights reserved.

iGEM '09 || TOP




Retrieved from "http://2009.igem.org/Team:NTU-Singapore/Notebook"