Team:NTU-Singapore/Notebook

Experiment Timeline

June

18th June

• Preparation of LB medium
• Amplification of parts from registry (pTet, TetR, HO1, Low copy plasmid)

19th June

• Antibiotic stock preparation
• Inoculation

20th June

• Our first Plasmid Extraction (PE)!

22nd June

• First Double Digestion (DD) and Gel Check!

23rd June

• PE & DD of the registry constructs….

24th June

• Our direct synthesized parts finally arrived. We now have CHE (cholesterol esterase), IFP(Infrared protein 1.4) and pNO (NO sensing promoter).

26th June

• PE , DD and Gel check for the direct synthesized gene sequence.

27th June

• Gel Check for CHE, IFP and pNO

July

1st July

• PE for plaqIQ, J23101, J23119, B0034(rbs), B0015(Term-T for short), B0034-GFP-B0015

2nd July

• PE for pNO, IFP, CHE
• DD of pNO, J23119, J23101, rbs-GFP-T
• Our first ligation!

3rd July

• DD of CHE, IFP and B0015, plaqIQ and plux.
• Ligation.

4th July

• PE: pNO-GFP; J23101-GFP; J23119-GFP; HO1
• DD: HO1