Team:USTC/Parts

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USTC
Home Team Project Modeling Parts Standard & Protocol Software Tool Human Practice Notebook

Team:USTC/Parts

Contents

To get the information of Parts, pleast visit [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2009&group=USTC USTC 2009 Parts]


This year we have submitted 171 biobrick, some of which were well characterized by a lot of measurements and analysised by modeling.

Here we seperated all the parts into different sets to describe according to our progress.
Assembly Road Map_1: the rudimentary map.
Assembly Road Map_2: the advanced map added the variation function.

1

' ? Name Type Description Designer Length
SentWorksRegulatorypLux/Tet Hybrid Promoter: (LuxR+,TetR-)->PoPSDanqian Liu, Chao Li, Hao Jiang72

2

' ? Name Type Description Designer Length
SentWorksReporterpLux/Tet(K176000)(LuxR+,TetR-)->RBS+GFP+TChao Li,Danqian Liu,Hao Jiang955

3

' ? Name Type Description Designer Length
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->PoPSDanqian Liu, Chao Li, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059
SentWorksSignallingTetR repressible AHL->PoPS Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1059

4

' ? Name Type Description Designer Length
UnsentIssuesTranslational_UnitPoPS->RBS+ccdB->PoPSZongxiao He, Hao Jiang324
SentIssuesTranslational_UnitPoPS->RBS+lacZalpha-ccdB->PoPSZongxiao He, Hao Jiang498
SentIssuesGeneratorPoPS->RBS+ccdB+TZongxiao He, Hao Jiang461
SentIssuesGeneratorPoPS->RBS+lacZalpha-ccdB+TZongxiao He, Hao Jiang635
SentIssuesTranslational_UnitPoPS->RBS(weak)+ccdB->PoPSZongxiao He, Hao Jiang326
SentIssuesTranslational_UnitPoPS->RBS(weak)+lacZalpha-ccdB->PoPSZongxiao He, Hao Jiang500
SentIssuesGeneratorPoPS->RBS(weak)+ccdB+TZongxiao He, Hao Jiang463
SentIssuesGeneratorPoPS->RBS(weak)+lacZalpha-ccdB+TZongxiao He, Hao Jiang637
SentIssuesCodingccdB-LVA coding sequenceDanqian Liu, Zongxiao He, Hao Jiang342
UnsentIssuesCodinglacZalpha-ccdB-LVA coding sequenceDanqian Liu, Zongxiao He, Hao Jiang513
SentIssuesTranslational_UnitPoPS->RBS+ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang360
SentIssuesTranslational_UnitPoPS->RBS+lacZalpha-ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang531
UnsentIssuesTranslational_UnitPoPS->RBS(weak)+ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang362
SentIssuesTranslational_UnitPoPS->RBS(weak)+lacZalpha-ccdB-LVA->PoPSDanqian Liu, Zongxiao He, Hao Jiang533
SentIssuesGeneratorPoPS->RBS+ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang497
SentIssuesGeneratorPoPS->RBS+lacZalpha-ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang668
UnsentIssuesGeneratorPoPS->RBS(weak)+ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang499
SentIssuesGeneratorPoPS->RBS(weak)+lacZalpha-ccdB-LVA+TDanqian Liu, Zongxiao He, Hao Jiang670

5

' ? Name Type Description Designer Length
SentWorksRegulatoryconstitutive promoter family member J23115 actual sequence (pCon 0.15)Hao Jiang, Danqian Liu, Chao Li35
SentWorksRegulatoryconstitutive promoter family member J23107 actual sequence (pCon 0.36)Hao Jiang, Danqian Liu, Chao Li35

6

' ? Name Type Description Designer Length
SentWorksReporterpCon max(J23119)->RBS+GFP+TChao Li,Danqian Liu,Hao Jiang918
SentWorksReporterpCon 1.00(J23100)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.70(J23101)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.36(K176009)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.15(K176008)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.04(J23109)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCon 0.01(J23103)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang918
SentWorksReporterpCI(R0051)(lambda CI-)->RBS+GFP+TChao Li, Danqian Liu, Hao Jiang932

7

' ? Name Type Description Designer Length
UnsentFailsGeneratorpCon max(J23119)->RBS+luxR+TChao Li,Danqian Liu,Hao Jiang979
SentWorksGeneratorpCon 0.70(J23101)->RBS+luxR+TDanqian Liu, Chao Li, Hao Jiang979

8

' ? Name Type Description Designer Length
SentNoneSignallingpCon max(J23119)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentNoneSignallingpCon 1.00(J23100)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.70(J23101)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.36(K176009)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.15(K176008)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.04(J23109)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841
SentWorksSignallingpCon 0.01(J23103)->RBS+luxI-LVA+TDanqian Liu, Chao Li, Hao Jiang841

9

' ? Name Type Description Designer Length
SentWorksSignallingTetR repressible AHL->GFP Receiver (Very High LuxR): pCon 0.70->luxR+pLux/Tet->GFPDanqian Liu, Chao Li, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (High LuxR): pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (Medium LuxR): pCon 0.15->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942
SentWorksSignallingTetR repressible AHL->GFP Receiver (Low LuxR): pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang1942

10

' ? Name Type Description Designer Length
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdBZongxiao He, Hao Jiang1528
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdBZongxiao He, Hao Jiang1702
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB(weak RBS)Zongxiao He, Hao Jiang1530
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Zongxiao He, Hao Jiang1704
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.36->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang1564
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVADanqian Liu, Zongxiao He, Hao Jiang1735
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1566
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.70->luxR+pLux/Tet->lacZalpha-ccdB-LVA(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1737
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
UnsentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.15->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1530
SentIssuesSignallingTetR repressible AHL->Death Receiver: pCon 0.04->luxR+pLux/Tet->lacZalpha-ccdB(weak RBS)Danqian Liu, Zongxiao He, Hao Jiang1704

11

' ? Name Type Description Designer Length
SentWorksGeneratorPoPS->RBS+tetR(no LVA)+TChao Li,Danqian Liu,Hao Jiang782
SentWorksGeneratorPoPS->RBS+tetR-LVA+TChao Li,Danqian Liu,Hao Jiang840

12

' ? Name Type Description Designer Length
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->GFPChao Li, Danqian Liu, Hao Jiang2732
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->GFPChao Li, Danqian Liu, Hao Jiang2790
SentWorksDeviceRepressible AHL->PoPS Receiver: PoPS->tetR+pCon 0.70->luxR+pLux/Tet->PoPSChao Li, Danqian Liu, Hao Jiang1849
SentWorksDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.70->luxR+pLux/Tet->PoPSChao Li, Danqian Liu, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
SentWorksDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.36->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.15->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790
UnsentFailsDeviceRepressible AHL->PoPS Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->PoPSDanqian Liu, Zongxiao He, Hao Jiang1907
UnsentFailsDeviceRepressible AHL->GFP Receiver: PoPS->tetR-LVA+pCon 0.04->luxR+pLux/Tet->GFPDanqian Liu, Zongxiao He, Hao Jiang2790

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