Team:Kyoto/CiC/Parts

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Parts

Part design

In this project, we designed two plasmid for RTS; 1. HIV-TAT::(LALAAAA)3 expressing vector 2. Signal for TIM23 complex::GFP expressing vector 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells. Both of 1 and 3 have T7 promoter, terminator and each CDS between them. They can express each protein by RTS (Rapidly Translation System, Roache). Both parts are inserted in high copy number plasmid pSB1A2. 3.Signal for TIM23 complex::EGFP expressing vector for HeLa cells is made by inserting Signal for TIM23 complex into pEGFP-N3.

HIV-TAT-(LALAAAA)3-generator

Fig.1

TIM23complex signal::EGFP generator

Fig.2


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