Major Results

This page contains highlights of our major results and simulations. It is by no means complete. However, more results will be presented at the jamboree.

Module 2 - Encapsulation

Wet lab experiments were performed to investigate growth of E. Coli cells under environments of different pH.

Module 3 - Genome Deletion

• The wet lab has also investigated the effects of restriction enzymes on cell population. Positive results were achieved.

The Dam negative strains are fully cleaved at all different concentrations of restriction enzyme. This produces the 'smear' that can be seen, as the DNA fragments are all of varying, but short lengths

From these results, we have shown that restriction enzymes can cleave Dam negative strains, hence killing Dam negative cells. This also proves Dam positive strains are protected again genomic deletion.

• Furthermore, the dry lab has provided a model on cell population under the production of restriction enzymes.

We have shown that an increase in restriction enzyme concentration will decrease total cell population, under the abscence Dam protection.

Module Integration - Autoinduction

• Experiments were performed on the CRP promoter. From this experiment, we are able to choose the best media for the optimal growth of cells.

Corrected fluorescence of glucose is almost negligible. From this, we have proved the theory that glucose represses the PcstA promoter strongly.

Also, 10% Casamino Acids in M9 shows the highest corrected fluorescence as well as a high corrected OD600, indicating good cell growth. Therefore, this is our media of choice.

diauxic growth

Module Integration - Thermoinduction

hvd gfp

References