Team:IIT Bombay India/Notebook

Week1 FACS Trial

Week2 (May, 18-23) Preparation

Week3 (May, 25-30) FACS

Week4 (Jun, 1-6) Growth Curve

Week5 (Jun, 8-13) FACS:YFP signal

Week6 (Jun, 15-20) FACS:CFP signal

Week7 (Jun, 22-27) FACS repeat

Week8 (Jun-Jul, 29-4) Req.:Transformation

Week9 (Jul, 6-11) Cultured Host

Week10 (Jul, 13-18) Transformation of 4 strains

Week11 (Jul, 20-24) FACS:Constructs

Week12 (Jul, 27-31) FACS

Week13 (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14 (Aug, 10-15) Confirmation of the transformants

Week15 (Aug, 17-22) Preparation for time course expression analysis

Week16 (Aug, 24-29) FACS time course

Week17 (Aug-Sep, 31-5) FACS: different IPTG conc.

Week18 (Sep, 7-13) Growth curves for all the four strains

Week19 (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20 (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21 (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22 (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23 (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)

Week24 (Oct, 18-21) Data compilation and analysis, wiki uploads