Team:IIT Bombay India/Notebook

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Week1 -FACS Trial

Week2 - (May, 18-23) Preparation

Week3 - (May, 25-30) FACS

Week4 - (Jun, 1-6) Growth Curve

Week5 - (Jun, 8-13) FACS:YFP signal

Week6 - (Jun, 15-20) FACS:CFP signal

Week7 - (Jun, 22-27) FACS repeat

Week8 - (Jun-Jul, 29-4) Req.:Transformation

Week9 - (Jul, 6-11) Cultured Host

Week10- (Jul, 13-18) Transformation of 4 strains

Week11- (Jul, 20-24) FACS:Constructs

Week12- (Jul, 27-31) FACS

Week13- (Aug, 3-8) Transformations in E.coli MG1655 (lac I deleted)

Week14- (Aug, 10-15) Confirmation of the transformants

Week15- (Aug, 17-22) Preparation for time course expression analysis

Week16- (Aug, 24-29) FACS time course

Week17- (Aug-Sep, 31-5) FACS: different IPTG conc.

Week18- (Sep, 7-13) Growth curves for all the four strains

Week19- (Sep, 14-20) Growth curve on Lactose (ptet A and plac K)

Week20- (Sep, 21-27) Growth curve on Lactose (plac A and ptet K)

Week21- (Sep-Oct, 28-3) Growth curve on Lactose varying IPTG concentrations (ptet A and plac K)

Week22- (Oct, 5-10) Growth curve on Lactose varying IPTG concentrations (plac A and ptet K)

Week23- (Oct, 12-17) Growth curve on Lactose (ptet A and plac K)

Week24- (Oct, 18-21) Data compilation and analysis, wiki uploads