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Team:UAB-Barcelona/PCRP
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Revision as of 23:46, 21 October 2009 by Antonio.freire (Talk | contribs)
PCR of nitrite detection sequences
We used GoTaq Green Master Mix (Promega)12
Annealing temperature
nirk (270 pb): as lowest temperature is 60ºC, the temperature we should set in the thermociclator would be 55ºC. But it didn't work properly at this temperature, so we set the temperature to 52ºC and 49ºC. PCR in both cases worked properly
Marker, 3x49ºC, controls, marker, 3x52ºC, controls
Annealing temperature
nsr(500 pb): the temperature is 76ºC, so we made the PCR at 71ºC. It didn't work. But when we tried with 65 and 68ºC it worked properly.
Marker, 4x65ºC, controls, marker, 4x68ºC, controls
