Team:UAB-Barcelona/CS1
From 2009.igem.org
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Cloning strategy
1st roundphoa (E,S) + backbone BBa_K093005 (E,S) // Biobrick 1 nirk (E,S) + backbone BBa_K093005 (E,S) // Biobrick 2 nsr (X,P) + backbone BBa_K093005 (E,S) // Biobrick 3
pLacI-RBS-RFP-T-T BBa_J04450 (E,S) + linker BBa_B0040 (E,X) // Biobrick 5 ptet-RBS-RFP-T-T BBa_I13521 (E,S) + linker BBa_B0040 (E,X) // Biobrick 6
RBS BBa_J61127 (S,P) + nsr (X,P) // Biobrick 8 ptet BBa_R0040 (S,P) + Biobrick 8 (X,P) // Biobrick 9 placI BBa_K091112 (S,P) + Biobrick 8 (X,P) // Biobrick 10
2nd roundBiobrick 4 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 11 Biobrick 7 (X,P) + RBS-RFP-T-T BBa_E0840 (S,P) // Biobrick 12 3rd roundBiobrick 11 + Biobrick 5 // Biobrick 13 Biobrick 11 + Biobrick 6 // Biobrick 14 Biobrick 9 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 15 Biobrick 10 (X,P) + RBS-GFP-T-T BBa_I13504 (S,P) // Biobrick 16
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