Team:DTU Denmark/results
From 2009.igem.org
Home | The Team | The Project | Parts submitted | Modelling | Notebook |
The redoxilator - Genetic design - Applications and perspectives - Results - Safety considerations The USER assembly standard - USER fusion of biobricks USER fusion primer design software - Abstract - Instructions - Output format |
The project In this section the project is presented. It is divided into four main sections that can be accessed to the left. Each section is briefly introduced below. The Redoxilator By in silico design and computer modelling followed by gene synthesis, we have constructed a molecular NAD+/NADH ratio sensing system in Saccharomyces cerevisiae. The sensor works as an inducible transcription factor being active only at certain levels of the NAD+/NADH ratios. By the coupling of a yeast optimized fast degradable GFP, the system can be used for in vivo monitoring of NAD+/NADH redox poise. Expected future applications of the system is heterologous redox coupled protein production in yeast, and a possible application in cancer research The USER fusion standard In this part of the project we propose a new parts-assembly standard for Biobricks based on USER cloning. The USER fusion assembly standard allows rapid construction of multi-part devices, without some of the drawbacks of the restriction-enzyme based standard biobrick assembly method. USER fusion primer design software When designing constructs with more than two biobricks using USER fusion, several parameters have to be taken into account when designing primers needed for the fusion. For this project we have programmed an online tool, that can automatically find the optimal primers for the fusion of up to 9 biobricks at once while taking several parameters into account including melting temperature, primer length and placement as well as avoidance of nearly identical primers. Experimental results Here the results of our experimental work is described including:
Besides the main project, 4 biobricks were made as a complement of our project and as a useful tool for the construction of future devices: |
Achievements |
Comments or questions to the team? Please Email us -- Comments of questions to webmaster? Please Email us |