Team:UNIPV-Pavia/Methods Materials/PlasmidExtraction

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Protocols


Plasmid extraction

(Estimated time: 3 hours if you use QIAGEN Plasmid Mini Kit; 1 hour if you use QIAGEN QIAprep Spin Miniprep Kit)

  • Follow QIAGEN standard protocol: [www.biochem.arizona.edu/AZ-START/graphics/QIAprep_Miniprep_Handbook.pdf QIAGEN QIAprep Spin Miniprep protocol]










  • 5 ml or 9 ml (for higher yields) overnight mini-culture
  • QIAGEN QIAprep Spin Miniprep Kit




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