Virginia Commonwealth/17 July 2009

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Contents

Friday 17 July 2009

Results

  • Results of overnight culture was successful
  • NEB 10 beta electrocompetency was successful
    • NEB on LB had lawn growth
    • NEB on LB after being shocked had lawn growth
  • Electrophoresis test on J06702 and pSB4C5
    • A DNA band was visible for both

Trentay 16:54, 17 July 2009 (UTC)

Craig and Clay

  • There is still no growth on the kanamycin and chloramphenicol plates.

Tasks

Maria and Afton

  • Religate promoters that did not grow in the first transformation (backbone pSB4C5)
    • J23101 w/ E0240
    • J23105 w/ E0240
  • Run an electrophoresis test on J06702 and pSB4C5

Trentay 15:53, 17 July 2009 (UTC)


Wetlab

Maria and Afton

  • Cryogenic stock of the following BioBricks was made (2 vials each)
    • J23101 w/ J06702
    • J23102 w/ E0240
    • J23102 w/ J06702
    • J23103 w/ E0240
    • J23103 w/ J06702
    • J23104 w/ E0240
    • J23104 w/ J06702
    • J23105 w/ J06702
    • J23107 w/ E0240
    • J23107 w/ J06702
  • Electrophoresis test

Trentay 15:53, 17 July 2009 (UTC)