Team:KULeuven/Notebook/Key/Lock/AntiKey

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Contents

Week 1: 6 July 2009 - 12 July 2009

Week 2: 13 July 2009 - 19 July 2009

[edit] Thursday

Plating the glycerol stock strain containing .

Week 3: 20 July 2009 - 26 July 2009

[edit] Wednesday

Liquid culture of has been made.

[edit] Thursday

The plasmid of has been isolated from the liquid culture. The glycerol stock strain containing has been plated.

[edit] Friday

The glycerol stock strain containing has been plated. The key+terminator sequence, the lock sequence and the antikey+terminator sequence have all been ordered at GeneArt.

Week 4: 27 July 2009 - 2 August 2009

[edit] Monday

Following parts were put in liquid medium:

  • + : GFP with LVA tag and terminators
    TetR-promoter

[edit] Tuesday

Following plasmids were purified:

  • GFP with LVA tag
    • concentration: 94,1 ng/μl
    • 260/280: 1,94
    tetR-promoter
    • concentration: 98,2 ng/μl
    • 260/280: 1,89

[edit] Wednesday

[edit] Thursday

[edit] Friday

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