"
Team:British Columbia/Project
From 2009.igem.org
| Home | The Team | The Project | Parts Submitted to the Registry | Modeling | Sponsor Us! | Biosensor Sensitivity Notebook | Biosensor Logic Gate Notebook | Bibliography |
|---|
Contents |
Overall project
1. Sensitivity of a biosensor
2. Logic gates
Biosensor
Using a random mutagenesis method directed at the promoter binding sites, we intend to construct a library of varying strength pBAD promoters coupled to a GFP reporter gene. Sequencing of select mutated promoters will result in a collection of pBAD variants that cover a wide range promoter sensitivity.
The Experiments
1. Random mutagenesis of pBAD promoter sequence. 2. Quantification of mutant promoter-driven GFP fluorescence. 3. Sequencing of select mutants. 4. BioBrick design and submission.
Results
Logic Gates
Miscellaneous Data
Biobricks.zip - Fasta file containing every biobrick from [http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=List Here]
Links
http://mobyle.pasteur.fr/cgi-bin/portal.py - a set of web-accessible bioinformatics tools including Mfold, which determines 2D RNA structure and draws it
http://frodo.wi.mit.edu/ - Primer3, a primer design program
