August/8 August 2009

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Today we carried out the following procedures (in rough chronological order):

1. Checked the cell cultures transformed and plated out yesterday (8/7) for colony formation and made an approximate count of the number of colonies.

(plate number)-(location on plate) (no. of colonies)

            2-12H                        none
            1-12C                        none
            1-12A                        none
            2-24G                        none
            2-18F                        none
                                               
            1-6O                         ~10
            1-10K                        ~10
            1-18L                        ~10

2. Conducted a 'miniprep' to harvest and check concentration of plasmids from 10 sample. (incubated since yesterday 8/7~) Result of Nanodrop concentration check:

(sample No.) (concentration) [260/280] [260/230]

    1-23L       24.9 ng/uL     1.77      1.77
    2-16F       36.0 ng/uL     1.77      1.99
    1-8O        19.3 ng/uL     1.65      1.59
    1-2M        18.4 ng/uL     1.64      2.01
    1-19B       40.9 ng/uL     1.56      0.83
    2-16H       27.7 ng/uL     1.68      1.38
    1-14D         9.3 ng/uL     1.66      2.31
    2-8M          9.9 ng/uL     1.65      1.90
    2-16M       24.6 ng/uL     1.72      1.24
    1-14F         2.5 ng/uL     1.11      0.25  ←×
    1-23J        ×

3. gel electrophoresis

	uL
EcoRⅠ	1
SpeⅠ	1
10*Buffer No.2	2
DNA (EpsE)	5
TOTAL	20

↓ 37℃,22hr ↓ ×　(SYBR Safe : 30min???)