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Team:Aberdeen Scotland/notebook/lacilatch
From 2009.igem.org
University of Aberdeen - Pico Plumber
iGEM 2009
LacI-Latch Notebook
Week 1: Research and Planning 08/06/09 - 12/06/09
Day 1 Monday (08/06/09)
- Researching the Registry for Biobricks
- Identified K182022, K112808, R0062, I712074, I14015 for further disccusion.
Day 2 Tuesday (09/06/09)
- Researching the Registry for Plasmids.
- Identified pSB3T5, pSB3C5, pSB1AC3, pSB1AT3 for further discusion.
Day 3 Wednesday (10/06/09)
- Literature search for Parameters of T7 promoter and the Holin system.
- Conlusion: T7 feedback mechanism of Wiring diagram 1 is unliekly to be functional without further regulations.
- Identified the novagen plasmids pLysS and pLysE as possible solutions.
- Researching the Registry for LacI Repressor and T7 Polymerase
- Identified K145014, K145001, I732100 for further discusion
Day 4 Thursday (11/06/09)
- Parameter search for the Modeling effort.
Day 5 Friday (12/06/09)
- End of week meeting
- Discussion and planning of following week
Week 2: BioBrick Rescue 15/06/09 - 19/06/09
Day 1 Monday (15/06/09)
1. Prepared LB medium for Lab 2. Planning of LacI-Latch 3. Inoculate J23100, K145001, B0034
Day 2 Tuesday (16/06/09)
1. Miniprep of J23100, K145001, B0034 2. Restriction Digest
Day 3 Wednesday (17/06/09)
1. Gel Electrophoresis of B0015, R0040, E0840, R0011, K093005, R0051, K081008, J37033, R0062,
Day 4 Thursday (18/06/09)
1. Gel Electrophoresis of K112808, K112022, I732100
Day 5 Friday (19/06/09)
1. Restriction Digest of E0840 2. Gel Electrophoresis of E0840
Week 3 22/06/09 - 26/06/09
Week 4 29/06/09 - 03/07/09
Week 5 05/07/09 - 10/07/09
Week 6 13/07/09 - 16/07/09
Week 7 20/07/09 - 24/07/09
Week 8 27/07/09 - 31/07/09
Week 9 03/08/09 - 07/08/09
Week 10 10/08/09 - 14/08/09
Week 11 17/08/09 - 21/08/09
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