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Uppsala-Sweden/11 August 2009
From 2009.igem.org
Evaluation of competent cells
Due to the run of bad transformations we've experienced we decided to evaluate the quality of our cells. We used our previosly extraxted Pbs1A (761 µg/ml) plasmid and as a control the pUC1G (100 pg/µl) plasmid from Invitrogen. The evaluation part of the protocol [http://openwetware.org/wiki/TOP10_chemically_competent_cells TOP10 chemically competent cells] was followed with the deviations noted:
For transformation we used 5 µl of 100 pg/µl plasmid instead of the 1 µl of 10 pg/µl as told by the protocol, the Pbs1A was diluted down to this concentration. This as this was what was given in the protocol supplied by Invitrogen.
20 and 200 µl of the transfomed cells were plated on 50 µg/ml ampicillin plates and icubated at 37 degrees over nigth.
--Anders 15:35, 11 August 2009 (UTC)
