AND Gate Notebook

Week 1: Research and Planning (08/06/09 - 12/06/09)

Day 1 Monday (08/06/09)

• Researching the Registry for Biobricks
  • Focus on T7 parts (I712074, I719005, K113011, K113012, J34814, K103021, R0085, R0180, R0182, Z0251 and Z0252) for possible use in our project.
  • Other BioBricks identified for other sub-projects include R0062, J40001, J37015 and R0063

Day 2 Tuesday (09/06/09)

• Researching literature for T7 promoter strength and degradation rate
  • Useful article - Imburgio, D., Rong, M., Ma, K., and W. T. McAllister. (2000) "Studies of Promoter Recognition and Start Site Selection by T7 RNA Polymerase Using a Comprehensive Collection of Promoter Variants" Biochemistry.39:10419-10430

Day 3 Wednesday (10/06/09)

• Researching literature for parameters of LuxR, LacO, TetO and cIO
  • Useful article - Braff, J. C., Conboy, C. C., and D.E. Endy. Promoter Characterization Experiments. (found at openwetware.org/images/3/30/PromoterChar_Report_Revised.doc)
• Identified plasmids that potential Biobricks were on and prepared for rescue.

Day 4 Thursday (11/06/09)

• PoPs to LacZ alpha repoter identified (E0435) and potenially useful Input Output sensor (pSB1A10)
  • Other possible reporters include LacZ alpha fragments and Enhanced stable YFP.
• Possibility of intentional SNP generated within T7 to alter promoter strength was deemed unworkable in the scale of project.

Day 5 Friday (12/06/09)

• End of week meeting
• Discussion and planning for following week

Week 2 15/06/09 - 19/06/09

Week 3 22/06/09 - 26/06/09

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09

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