Uppsala-Sweden/14 August 2009

From 2009.igem.org

Revision as of 14:29, 14 August 2009 by Karl.brune (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Transformation of Adh2 z, evaluation of competence

continued since yesterday

So, it's official, our competent cells are really quite incompetent! Tested against the new bath of competent DH5alfa which got 220 transformations (100*10^6 / µg pUC 19)our perfored extremly bad at around 20 transformations.

We will make new CCMB80 (unauotclaved this time) and also consider to switch cells to TOP10 instead.

--Karl.brune 14:28, 14 August 2009 (UTC)

Plasmid prep contiued

Yesterday we performed a plasmid prep on all transformed genes. Today I did the same for the B0034 RBS. This with a small screw up as I accedentilly eluted with 1 M Tris-HCl as opposed to 5 mM! I precipitated the DNA wizh ethanol and resuspen in 5 mM Tris-HCl.

Left to do: Measure DNA koncentration for all the plasmid preps.

--Karl.brune 14:28, 14 August 2009 (UTC)