August/14 August 2009

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miniprep

Conducted a "miniprep" to harvest and check concentration of plasmids from 14 sample.Result of Nano drop concentration check :(sample No.) (concentration) [260/280] [260/230]

  1-12C  29.5ng/μL　 1.56  0.91
  1-6I   7.5        1.88  2.79
  1-12A  11.5       1.92  2.65
  1-18L  28.6       1.81  2.46
  1-2M   10.1       1.96  2.60
  1-16G  14.0       1.99  2.47
  2-8I   15.4       1.86  3.29
  2-21F  17.9       1.99  2.60
  2-11N  11.2       1.88  2.32
  2-24G  19.5       1.97  2.34
  2-8O   11.0       1.60  1.74
  2-6E   5.5        1.46  2.03
  1-3    16.1       1.78  2.40
  1-8    14.4       1.77  2.38

transformation

to get new plasmid

   sample No.
    ③　④　⑤　⑥