| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| pSB1A2 | ech + rbs | purified plasmid (oranje) | | 108,8 ng/µl | August 4
|
| pSB1A2 | fcs + rbs | purified plasmid (oranje) | | 113,9 ng/µl | August 4
|
| pSB1A2 | Sam5 + rbs | received from Edinburgh | | | August 3
|
| pSB1A2 | Sam5 + rbs, PstI site removed | purified plasmid (oranje) | | 101,3 ng/µl | August 5
|
| pSB1A2 | Sam8 + rbs | purified plasmid (oranje) | | 95,7 ng/µl | August 5
|
| pSB1A2 | Sam8 (b) | purified plasmid (oranje) | | 81,5 ng/µl | August 5
|
| pSB1A2 | COMT | purified plasmid (oranje) | | 122,9 ng/µl | August 5
|
| pSB1A2 | COMT + rbs | plasmid from registry | 0 µl | | August 6
|
| pSB1A2 | ech + rbs | purified digest (C2) | | 16,9 ng/µl | August 6
|
| | fcs + rbs | purified digest (C1, FCS Rb) | | 16,6 ng/µl | August 6
|
| pSB1A2 | sam5 + rbs | purified digest (A2) | | 3,1 ng/µl | August 7
|
| | sam8 + rbs | purified digest (A1) | | 3,4 ng/µl | August 7
|
| pSB1A2 | sam5 + sam8 | ligation (lig. A) | | | August 11
|
| pSB1A2 | ech + fcs | ligation (lig.C) | | | August 11
|
| pSB1A2 | fcs + rbs | purified plasmid (fcs pl.) | 39 µl | 57,2 ng/µl | August 12
|
| pSB1A2 | Sam5 + rbs | purified plasmid (sam5 pl.) | 41 µl | 76,0 ng/µl | August 12
|
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 0 µl | 45,2 ng/µl | August 12
|
| | fcs + rbs | purified digest (F rd.) | 28 µl | 4,2 ng/µl | August 12
|
| pSB1A2 | sam5 + rbs | purified digest (5 rd.) | 28 µl | 7,3 ng/µl | August 12
|
| pSB1A2 | ech + rbs | purified plasmid (ech pl.) | 48 µl | 72,7 ng/µl | August 13
|
| pSB1A2 | Sam8 + rbs | purified plasmid (sam8 pl.) | 48 µl | 66,2 ng/µl | August 13
|
| pSB1A2 | sam5 + sam8 | cells with sam5+sam8 (elek. A) | 600 µl | | August 13
|
| pSB1A2 | ech + fcs | cells with ech+fcs (elek. C) | 600 µl | | August 13
|
| | sam8 + rbs | purified digest (sam8 rb.) | 26 µl | 9,4 ng/µl | August 14
|
| pSB1A2 | ech + rbs | purified digest (ech rb.) | 26 µl | 13,6 ng/µl | August 14
|
| pSB1A2 | sam8 + Sam5 | ligation (SAMS) | 28 µl | | August 14
|
| pSB1A2 | ech + fcs | ligation (EF) | 28 µl | | August 14
|
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| 2235 | / | forward primer for | | | 20μM | August 3
|
| 2238 | / | reverse primer for | | | 20μM | August 3
|
| 2235 | / | forward primer for | | | 100μM | August 3
|
| 2238 | / | reverse primer for | | | 100μM | August 3
|
| 2242 | / | forward primer for | | | 20μM | August 3
|
| 2243 | / | reverse primer for | | | 20μM | August 3
|
| 2242 | / | forward primer for | | | 100μM | August 3
|
| 2243 | / | reverse primer for | | | 100μM | August 3
|
| 2244 | / | forward primer for | | | 20μM | August 3
|
| 2245 | / | reverse primer for | | | 20μM | August 3
|
| 2244 | / | forward primer for | | | 100μM | August 3
|
| 2245 | / | reverse primer for | | | 100μM | August 3
|
| 2246 | / | forward primer for | | | 20μM | August 3
|
| 2247 | / | reverse primer for | | | 20μM | August 3
|
| 2246 | / | forward primer for | | | 100μM | August 3
|
| 2247 | / | reverse primer for | | | 100μM | August 3
|
| 2288 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2288 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2289 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2290 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2291 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2292 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2293 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 20μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 20μM | August 8
|
| 2294 | / | forward primer for mutagenesis for | | | 100μM | August 8
|
| 2295 | / | reverse primer for mutagenesis for | | | 100μM | August 8
|
miscellaneous
| part
| plasmid
| description
| status
| μl left
| concentration
| date
|
| dNTP mix | | ATP, GTP, TTP, CTP | | 94 | 10μM | aug 10
|
| pSB1A2 | medium constitutieve promoter | | | | july 23
|
| ampicilline | | | | | |
|
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