Uppsala-Sweden/19 August 2009

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Preparing competent cells

Continued since yesterday

We harvested the seed cultures from yesterday and will later inoculate 300 ml of SOB media with one seed culter for harvest tomorrow. We also put three seed stocks in the -80 freezer for future use.


Gel run

A gel run was performed to evaluate one of the PCR runs from yesterday

Setup: Media:Example.jpg

Result: Example.jpg

Another gel run was started to evaluate the other PCR performed yesterday.......


Tansforming ADH2 Z.mobilis

Continued since yesterday

The results form the "on column" digested ADH" was good with many colonies on the 50µl plate. We picked 7 of them and started a Taq evaluation PCR

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