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CAROL
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WIKI CODING HERE
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CHINMOYEE
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EMILY
Construction Begins!
Now that we have our gene of interest, LuxOD47E, Biobricked, we can start constuction to add the J13002 promoter and the B0015 terminator. We'll do two constrcutions in parallel and see if one of them works.
* Did a restriction digest cutting the insert with EcoRI and SpeI and cutting the recipient with EcoRI and XbaI restriction enzymes.
* Performed Antarctic Phosphotase Treatment (NEB) on the recipients, followed by ligation of the inserts and recipients.
*Transformed J13002-LuxOD47E into TOP10 cells and plated on AC and C plates in 25 uL and 50 uL, left plates for overnight growth.
*Did restreaks and made overnight cultures of colony2X LuxOD47E BBK.
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
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KEVIN
1. Plasmid Isolation of fluorescent proteins
Isolation of fluorescent proteins were done in order to verify the presence and functionality of fluorescent proteins and promoters.
1. Testing of fluorescent proteins and the promoters
Isolated Flurorescent proteins were constructed behind of R0040 or J13002, depending on whether or not the fluorescent protein contained RBS, and transformed into TOP10 cells.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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