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Uppsala-Sweden/19 August 2009
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Revision as of 16:45, 19 August 2009 by Karl.brune (Talk | contribs)
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Preparing competent cells
Continued since yesterday
We harvested the seed cultures from yesterday and will later inoculate 300 ml of SOB media with one seed culter for harvest tomorrow. We also put three seed stocks in the -80 freezer for future use.
Gel on Taq grad PCR with pirA BB primer
I made a new gel of the Taq PCR yesterday.
--Karl.brune 16:45, 19 August 2009 (UTC)
Gel on the Phusion PCR on adh2(Y) and pirA (BB & grad)
--Karl.brune 16:45, 19 August 2009 (UTC)
Gel on Taq grad PCR with pirA primer variations and Colony PCR
--Karl.brune 16:45, 19 August 2009 (UTC)
Incubation of #3 adh2(Z)
- 3 adh2(Z) in 5ml LB + amp
incubated overnight @37°C and 250rpm
--Karl.brune 16:45, 19 August 2009 (UTC)
Incubation of competent cells
1ml in 300ml of LB --Karl.brune 16:45, 19 August 2009 (UTC)
Tansforming ADH2 Z.mobilis
Continued since yesterday
The results form the "on column" digested ADH" was good with many colonies on the 50µl plate. We picked 7 of them and started a Taq evaluation PCR
