Since it is very hard to find relavent kinetic constants ( these constants change with different conditions)we decided to find an order of magnitude for our kinetic constants in certain situations and the rest of the precision we hope to optimize at a certain point in the future.
We looked through many papers and fortunately we managed to find some magnitudes for the dephosphorylation and phosphorylation rate. The smaller the kinetic rates the longer the reaction time.
We also looked into finding the precision and limitations of the fluoresecence reading instrument. The protocol was discussed and preparations were made so that the instrument could be tested tomorrow.
Issues of disscussion :
Are all reactions really elementary ? How accurate is it to assume that the order of magnitude of the kinetic equations is determined by the number of species present in the reactants ?
After some research into paper :
One other math modelling paper looked into modelling the interactions of insulin . The autophosphorylation of (X...), receptor dephosphorylation and degradation of insulin was simply assumed to be first order kinetics.
I did some research into the research of GFP productuion
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