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CAROL
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WIKI CODING HERE
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CHINMOYEE
CLASS
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EMILY
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FAHD
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IMAN
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JAMIE
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JEREMY
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KATIE
Back to the Lab after Completing Initial Version of Quorum Sensing
Quorum sensing bacteria are complete and I made bacteria with RFP and placed them beside other bacteria for display. I will probably return to work on this later, but it is now more important to finish the lab. I learned that the order in which you add materials during restriction digest is important so using the function (initially from cleaning script) created for storing the names of items in an inventory will be important to make sure that each time something is added, it is the correct item. I believe for the rest of the activity I may be able to use a modified version of my find item script.
However, I have yet to determine a new way to move things to the water bath, heating block etc. in a creative way and still keep avatars involved.
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KEVIN
1. Plasmid Isolation of R0040, B0015, and J13002
R0040 (promoter), B0015 (terminator), and J13002 (RBS+Promoter) were isolated in order to verify the presence of them with Restriction digest. These parts are needed for general construction of our circuits.
2. Restriction digest of R0040, B0015, and J13002
To verify if B0015, R0040, and J13002 are present within the plasmid/cell, restriction digest was performed using NotI enzyme.
The band in R0040 C2 #1 is the only one that did not match the expected size of each. Only one colony is needed to move on.
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MANDY
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PATRICK
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PRIMA
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STEFAN
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VICKI
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