Quorum Sensing Notebook

Week 1 - Research and Biobrick Rescues (08/06/09 - 12/06/09)

Day 1 Monday (08/06/09)

• Researching the Registry for Biobricks
  • Focus on QS module
  • Identified K081008, J23100, C0062, K091156, B0034,B0030, B0015, R0061 and R0062 for possible use in our project.
  • Other biobricks identified for other sub-projects include R0011.

Day 2 Tuesday (09/06/09)

• Researching the literature for information on Quorum Sensing.
• Researching for information on LVA tags

Day 3 Wednesday (10/06/09)

• Preparing for biobrick rescue and transformation
  • Prepare LB and Agar
  • Prepare solutions
    • Cacl2 solution,
    • 1M MgCl2, 1MMgSO4 and 2M glucose solutions (for SOC medium)
  • Inoculate e.coli and grow overnight

Day 4 Thursday (11/06/09)

• Rescue Biobricks (K081008, J37033, B0015, J23100, R0011, R0062, K112808, C0051, C0040, I732100, R0051, K145001 and B0034)
• Prepare competent e.coli, using calcium chloride method
• Transform e.coli with biobricks listed above
  • grow overnight on agar/amp plates.

Day 5 Friday (12/06/09)

• Results from yesterday indicate that only I732100 was successfully transformed into e.coli
• Identify possible recipient plasmids for QS module (psB4C5).
• Propose a cloning strategy
• Team meeting

Week 2 15/06/09 - 19/06/09

Week 3 22/06/09 - 26/06/09

Week 4 29/06/09 - 03/07/09

Week 5 05/07/09 - 10/07/09

Week 6 13/07/09 - 16/07/09

Week 7 20/07/09 - 24/07/09

Week 8 27/07/09 - 31/07/09

Week 9 03/08/09 - 07/08/09

Week 10 10/08/09 - 14/08/09

Week 11 17/08/09 - 21/08/09

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