Template:Team:KULeuven/11 August 2009/VanillinReceptor

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Revision as of 12:33, 25 August 2009 by Didierborowski (Talk | contribs)
  • We achieved good results for genes B, G and R. There were clear white colonies after plating the competent cells with the TOPO vector on X-gal agar plates with ampcillin. We isolated the good colonies again.
  • There was no result for A. This was probably due to a fault with the competent cells. So we started TOPO transformation by heat shock all over for A.
  • We also recieved another strain of Agrobacterium. We brought this new strain in culture.
  • From now on to make things clear the genes from the first Agrobacterium will be called A,B,G and R for respectively virA, promotor regin virB , virG and rpoA. For the second agrobacterium we will be using W,X,Y and Z as name for the same genes.
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