Template:Team:KULeuven/25 August 2009/BlueLightReceptor

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Revision as of 13:47, 25 August 2009 by AnneV (Talk | contribs)
    • miniprepping the liquid cultures with pSB3K3
Part concentration (ng/μl) 260/280 λ
pSB3K3 (1) 27,6 1,91
pSB3K3 (2) 47,7 2,08
    • restriction digest of pSB3K3 with EcoRI and PstI
    • gel electroforesis and extraction of the RD of pSB3K3

--> failed. there was no signal at all

    • new colonies were ented in liquid culture.
    • ligation C performed: J23101 (A) + E0240