Template:Team:KULeuven/17 August 2009/VanillinReceptor

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  • A failed again so we decided to work only with W instead of performing a new PCR. TOPO for W and Z worked because there were colonies visible. White colonies were plated out.
  • Fluid cultures of G2 and G3 were miniprepped, nanodropped and cut with Dpn I to exclude the motherstrain
  • Mutagenesis 2 on R on position 427 was performed with PCR
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