Template:Team:KULeuven/26 August 2009/BlueLightReceptor

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  1. the plates with ligation A (blp + GFP) where fetched from the blue light installation. there was no GFP signal. the following actions will be taken:
    • the plasmids will be purified from the colonies and will be sequenced using primer 2260.
    • next time we will probably put them in liquid cultures under the LEDs while shaking gently. also, other parameters that need to be considered are being researched.
    • they will not be exposed to the light as long anymore. we decided that 1h will be more then enough.
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